• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:

    公开号:SU1015834A3
    申请号:SU802937820
    申请日:1980-06-23
    公开日:1983-04-30
    发明作者:Бляйштайнер Манфред；Риттерсдорф Вальтер；Вилингер Ханс
    申请人:Берингер Маннхайм Гмбх (Фирма)；
    IPC主号:
    专利说明:

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    The invention relates to a persistent fast indicator, which serves for the semiquantitative determination of ascorbic acid in beverages and body fluids. An indicator for determining ascorbic acid, which consists of a mixture of neutral nitrate and phosphorolybdic acid and malonic acid 1, is known. A disadvantage of the known indicator is low storage stability, associated with the fact that malonic acid, during storage, sublimates, which reduces the accuracy of the analysis of ascorbic acid.
    The purpose of the invention is to increase the storage stability and increase the accuracy of determination.
    The goal is achieved by the fact that the indicator for the determination of ascor: binic acid as a salt of phosphormolybdic acid contains a salt of an alkali metal of 2.18-phosphormolybdic acid, an alkali metal chlorate, citric or block acid, or tartaric acid, or a mixture of these acids with their alkali metal salts in the following ratio of components, water or water-me .tanol solution:
    Salt of alkaline-methylgipla 2,18-phosphormolybdic acid 3-9
    Alkaline chlorate
    metal1 b
    Lemon or block, or tartaric acid, or a mixture of these acids with their alkaline salts.
    metal / 10-90 Strips of indicator paper are made as follows.
    The alkali metal salt of 2.18-phosphorimolybdic acid, at a concentration of 3.0-9.0 g / l, is dissolved in water along with 1.0-6.0 g / l of alkali metal chlorate and 10-90 g / l of aliphatic hydroxycarbonate acids with the addition of any organic solvent or lower order alcohol that is mixed with or without water to the solution. Using caustic alkali, for example, lithium hydroxide or sodium, etc., the pH of the solution is adjusted to 2.5 to 5.0.
    A mixture of hydroxycarboxylic acid with the corresponding alkali metal salt is also used, and the ratio of the components of the mixture is chosen in such a way that the pH value is 2.5-5.0. Mixtures of organic acids are also used. These impregnating solutions in the usual way impregnate the filter. After drying, the prepared reactive paper is cut and then further processed to produce individual or combined test strips, for example, by sticking onto a plastic or film holder or wetting. Using the test strips made in this way, the content of ascorbic acid in liquids is determined either manually, by comparing the color of the plates, or by the method of viscosity photometry.
    Example. 7 g of sodium salt of 2,18-phosphorimolybdic acid, 4 g of sodium chlorate, 40 g of citric acid and 13.5 trilithium citrate are dissolved in a mixture of 600 ml of methanol and 380 ml of water. This solution, the pH of which is 3.7, is used to impregnate the filter paper and then dry it for 1 hour at. From the reactive paper prepared in this way, the pieces are sized 6x6 mm, which are then sealed between the plastic holder and the thin nylon mesh. These test strips are very good; they are painted in colors from light green-blue to dark-blue-violet with ascorbic acid in the urine in the range of very important concentrations from 0 to 40 mg / l.
    The indicator produced in this way is also suitable for the semi-quantitative determination of ascorbic acid in fruit juices or other beverages, and in this case it is necessary to dilute it with water before final determination. Objective assessment of the reaction color is set using a photometer.
    In tab. Figure 1 shows the luminance values measured using a DMK 21 spectral photometer with a nozzle: Zeiss ZR21 from the company, and the color of the color detected by the eyes.
    EXAMPLE 2. 6.5 g of sodium salt of 2.18-phosphoE of olibdic acid 4 g of sodium chlorate and 45 g of malic acid or tartaric acid are dissolved in water and the pH of the solution is adjusted to 10 with caustic alkali. , 5-5.0. Further processing to obtain indicator strips is performed as in Example 1. The functions and properties of the indicator strips correspond to the functions and properties described in Example C.
    EXAMPLE 3 Reactive paper according to Example 1 or 2 is combined into multi-layer test strips together with reactive papers for determining nitrate, pH, glucose and ketone bodies, which are made by a known method. These multi-layered strips are stored in closed tubes for several weeks. For a comparative test, reactive paper strips of Example 1 are made, the composition of the components of which is changed so that instead of citric acid and lithium citrate, equal amounts of succinic acid or malonic acid and lithium hydroxide are added to obtain a pH of 3.6. These reactive strips of paper are also treated as described above for the manufacture of multi-layer strips and are stored in an equal manner for several times. If the experiment is carried out using multi-layer strips, the composition of which corresponds to the invention, then a true pH value is obtained, for example for urine. If the test wire with multilayer strips that contain an indicator of ascorbic acid, made with amber acid, then the pH value is wrong. The same negative effect on the pH-indicator can be observed, if the paper is impregnated with a salt of phosphomolybdic acid and malonic acid, and stored for several weeks Prler4. Gg of the potassium salt of g, 18-phosphorus of 1-molybdic acid, 1 g of potassium chlorate and 10 g of tartaric acid are dissolved in water and the pH of the solution is adjusted to Yun. soda liquor at 2.5-5.0. Further processing for the test is carried out as in Example 1. The effect and properties of the test strips are studied by placing in asprobinic acid with a concentration of 0-100 mg / 100 ml. Excess liquid is removed from the edge of the vessel and staining is determined after 1-2 minutes of reaction time. . The test strips treated in this way are inserted for
    Concentration of asc orbic acid, mg / 80
    Brightness at, 640 nm,%
    The color of the quantification in remission photometer and remission is determined at 640 nm-max. INTENSITY of the blue dye produced as compared with the bellevum standard (see Table 2). Subjective determinations are determined by eye due to a good gradation of semi-quantitative. The concentration of ascorbic acid of unknown solutions is measured in a similar way and determined by a calibration curve or a subjective comparison. ExampleB 9 g of lithium salt of 2.18 phosphomolybdic acid, 6 g of sodium chlorate, 45 g of citric acid, and 45 g of sodium citrate are dissolved in water. The pH of the solution is set to 10 n. soda liquor on 2,5-. 5.0. Further processing is carried out as described in Example 1. The function and properties of the test strip are examined. To do this, it is immersed in ascorbic acid with a concentration of 0-100 mg / ml. Excess liquid is dropped from the edge of the vessel and the color is determined after 1-2 minutes of reaction time. Quantitative determination is performed by immersing the test strip thus treated using a remission photometer. The remission is determined at an AD of 640 nm — the most intense blue color in comparison with the white standard (see Table 3). A subjective assessment provides a semi-quantitative, thanks to a good gradation, visual definition. The concentration of ascorbic acid of unknown solutions is measured in a similar way by comparison with a calibration curve or by subjective assessment when compared with comparative colors. Table 1
    ABOUT
    five
    ten
    20
    40
    100
    Pale yellow
    Light green
    Green-bare ba
    Goluba
    Blue violet
    Dark blue and purple
    权利要求:
    Claims (1)
    [1]
    INDICATOR FOR DEFINITION. Ascorbic acid, containing a salt of phosphormolybdenum acid and an organic acid, distinguished by the fact that, in order to increase storage stability and increase the accuracy of determination, it contains, as a salt of phosphormolybdenum acid, an alkali metal salt of 2.18-phosphormolybdenum acid, in as an organic acid - citric or malic, or tartaric acid, or a mixture of these acids with their alkali metal salts and additionally contains alkali metal chlorate in the following ratio of components, g / l aqueous or aqueous methanol solution: Alkali metal salt of 2,18-phosphoformolybdic acid 3-9
    Alkali metal chlorate 1-6
    Citric or malic or tartaric acid or a mixture of these acids with their alkali metal salts. 1
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    同族专利:
    公开号 | 公开日
    DE3062883D1|1983-06-01|
    JPS568550A|1981-01-28|
    YU167480A|1984-04-30|
    US4300905A|1981-11-17|
    JPS6321865B2|1988-05-09|
    EP0021245A1|1981-01-07|
    AT3169T|1983-05-15|
    CS221928B2|1983-04-29|
    EP0021245B1|1983-04-27|
    DE2926068A1|1981-01-08|
    DD151820A5|1981-11-04|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US3771964A|1972-02-28|1973-11-13|Miles Lab|Test composition and device for ascorbic acid determination|
    US3825411A|1972-08-30|1974-07-23|Medico Electronic Inc|Reagent and method for bilirubin determination|
    JPS53712A|1976-06-24|1978-01-06|Kansai Chubu Kk|Surface treatment method of vivifying embossed and etched sections on metal article|DE4304728C2|1993-02-13|1997-04-10|Igor Dr Popov|Method and test kit for the determination of ascorbic acid in biological samples|
    EP0833161A1|1996-09-23|1998-04-01|Alfred B. Ordman|Method for maintaining a continuously-saturated level of ascorbic acid in a patient's body|
    US6221614B1|1997-02-21|2001-04-24|The Regents Of The University Of California|Removal of prions from blood, plasma and other liquids|
    US6620629B1|1997-02-21|2003-09-16|The Regents Of The University Of California|Method for detecting prions|
    US6719988B2|1997-02-21|2004-04-13|The Regents Of The University Of California|Antiseptic compositions for inactivating prions|
    US6617119B2|1997-02-21|2003-09-09|The Regents Of The University Of California|Assay for specific strains of multiple disease related conformations of a protein|
    US6720355B2|1997-02-21|2004-04-13|The Regents Of The University Of California|Sodium dodecyl sulfate compositions for inactivating prions|
    US20060008494A1|1997-02-21|2006-01-12|The Regents Of The University Of California|Complete inactivation of infectious proteins|
    US6841060B2|1999-05-20|2005-01-11|Shanbrom Technologies, Llc|Method for quantifying antioxidant levels in food and medical specimens|
    US20080003629A1|2006-04-07|2008-01-03|Morris Shayne K|Device and method for detection of vitamins and nutritional minerals|
    JP6874133B2|2016-10-28|2021-05-19|シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレイテッド|Detection of ascorbic acid in urine samples|
    CN110114673B|2016-12-19|2020-10-20|优卡喜公司|Urine detection device and urine detection method|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    DE19792926068|DE2926068A1|1979-06-28|1979-06-28|QUICK TEST FOR DETECTING ASCORBIN ACID|
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